Welcome to MAP2.03D Server
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Sequence based analysis
(Please provide a nucleotide sequence as input and submit the job)
 
Paste nucleotide sequence belowPaste raw nucleotide sequence or in fasta, GCG or Genbank format
 
Or upload sequence file Upload the file of raw nucleotide sequence or in fasta, GCG or Genbank format  example file
 
 Define mutagenesis method* MAP analysis performed upon 19 commonly used random mutagenesis methods. Please click here to include another mutagenesis method in MAP analysis.
 

*Please mouse over the input labels for help!

Processing... Do not refresh the page!

Structure based analysis
(Please provide the PDB file along with the nucleotide sequence as input and submit the job)
 
Upload PDB fileUpload the crystallographic structure or homology model of the protein (Identity should be more than 80% for the best results)  example PDB           ChainPlease enter the chain id in case of multimer structure 
 
Select methodPlease select one random mutagenesis method from the dropdown menu (*Default is Non biased)
 
Select the amino acid groupPlease select one of the amino acid group (categorized according to their chemical properties)

*Please select 'amino acid list' option to perform analysis upon a set of amino acids
(*Default is for all residues)
 
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Define molecular interaction parameters*To change the default parameters for calculation of molecular interaction

*Please mouse over the input labels for help! **Please don't refresh the page, the query will take a while to be completed for large proteins!
Reference: Verma R, Schwaneberg U, Roccatano D 2012. MAP2.03D: A sequence/structure based server for protein engineering  
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