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Input sequence
 Number of nucleotides: 1107
 Number of amino acids: 369
 GC content of the sequence: 62.51
 
MAP results
(click on links for the content and mouseover on links for description)
a.  Protein structure indicatorThe structure/function disrupting (stop codons) and likely destabilizing (glycine and proline residues) amino acid substitutions
    1.  Fraction of variants with stop codonsFraction of single nucleotide substitutions resulting in a stop codon
    2.  Fraction of variants with Gly or ProFraction of single nucleotide substitutions resulting in a glycine or proline codon
b.  Amino acid diversity indicatorThe fraction of variants with preserved amino acids and average amino acid substitution per residue after single nucleotide exchange
    1.  Fraction of variants with preserved amino acidsFraction of single nucleotide substitutions that do not change the encoded amino acid
    2.  Average amino acid substitution per residueAverage number of amino acid substitutions after single nucleotide exchange of a codon
    3.  Codon diversity coefficientThe distribution of random mutations among codons of a gene
c.  Chemical diversity indicatorAnalyse the chemical diversity generated by the random mutagenesis method
*For this analysis amino acids are grouped into four groups (aliphatic, aromatic, neutral and charged) along with stop codons

    1.  Amino acid substitutionsThe percentages of stop codon, charged neutral, aromatic,and aliphatic amino acid substitutions generated by random mutagenesis methods
    2.  Deviation from chemical distributionReport the deviation from chemical distribution of the sequence for each random mutagenesis method
    3.  Amino acid substitution patternsThe amino acid substitution patterns for mutagenesis methods


(The structure/function disrupting (stop codons) and likely destabilizing (glycine and proline residues) amino acid substitutions. Bar colors show the mutagenesis method employed for nucleotide substitution: enzyme based methods in red, whole cell methods in green, synthetic chemistry based methods in blue and theoretical nonbiased method in black colour.)
Fraction of variants with stop codons Fraction of variants with Gly or Pro
this is a star this is a star


(The fraction of variants with preserved amino acids and average amino acid substitution per residue after single nucleotide exchange. Bar colors show the mutagenesis method employed for nucleotide substitution: enzyme based methods in red, whole cell methods in green, synthetic chemistry based methods in blue and theoretical nonbiased method in black color.)
Fraction of variants with preserved amino acids Average amino acid substitution per residue
this is a star this is a star


(Codon diversity coefficient shows the distribution of random mutations among codons of a gene for mutagenesis methods (enzyme based methods in red, whole cell methods in green, synthetic chemistry based methods in blue and theoretical nonbiased method in black color). Chemical diversity indicator shows the percentages of stop codon (red), charged (pink), neutral (yellow), aromatic (cyan) and aliphatic (blue) amino acid substitutions generated by random mutagenesis methods.)
Codon diversity coefficient Amino acid substitutions
this is a star this is a star


(The values represent the deviation from the chemical distribution of the sequence for random mutagenesis methods)
Methods/Amino Acid Classes Stop Charge Neutral Aromatic Aliphatic
Chemical distribution 0.00 % 25.00 % 27.99 % 7.34 % 39.67 %
Non-biased 4.03 % -2.06 % 3.45 % -0.83 % -4.59 %
Taq (-. G>A=C=T) 2.18 % -2.45 % 5.62 % -3.00 % -2.34 %
Taq (+. G=A=C=T) 2.30 % -0.34 % 3.37 % -3.19 % -2.13 %
Taq (+. G>A=C=T) 3.48 % -1.84 % 4.19 % -1.58 % -4.25 %
Taq (+. G>>A=C=T) 1.51 % -0.04 % 3.98 % -4.36 % -1.10 %
Taq (+. G=A. C=T) 4.38 % -2.62 % 4.47 % -0.23 % -6.00 %
Taq (+. G=T. A=C) 2.43 % -0.80 % 3.69 % -2.78 % -2.55 %
Taq (I614K) 3.65 % -1.84 % 3.86 % -1.00 % -4.68 %
Mutazyme I 5.22 % -2.72 % 2.90 % 1.14 % -6.54 %
Mutazyme II 5.09 % -2.74 % 3.52 % 0.75 % -6.62 %
Pfu (exo-. D473G) 4.59 % -2.52 % 3.72 % 0.12 % -5.92 %
Transcriptase 4.31 % -1.79 % 4.92 % -1.13 % -6.31 %
Taq (dPTP/8-oxodGTP) 1.15 % -0.10 % 3.94 % -4.58 % -0.41 %
epRCA 5.26 % -3.15 % 3.10 % 1.76 % -6.98 %
Pol I 4.26 % -2.50 % 3.62 % 0.21 % -5.59 %
E. coli (mutA) 5.34 % -3.12 % 3.49 % 0.97 % -6.68 %
Nitrous acid 4.51 % -7.10 % -4.83 % 4.69 % 2.73 %
Formic acid 4.86 % -7.06 % 8.83 % 1.43 % -8.05 %
Hydrazine 2.50 % -5.78 % 1.88 % -0.05 % 1.44 %
EMS 3.15 % -1.19 % 2.94 % -1.28 % -3.63 %


Amino acid substitution patterns

(The amino acid substitution pattern for mutagenesis methods. The Y-axis shows the original amino acid species and the X-axis shows the substitution pattern.)
Non-biased this is a star Taq (-. G>A=C=T) this is a star
Taq (+. G=A=C=T) this is a star Taq (+. G>A=C=T) this is a star
Taq (+. G>>A=C=T) this is a star Taq (+. G=A. C=T) this is a star
Taq (+. G=T. A=C) this is a star Taq (I614K) this is a star
Mutazyme I this is a star Mutazyme II this is a star
Pfu (exo-. D473G) this is a star Transcriptase this is a star
Taq (dPTP/8-oxodGTP) this is a star epRCA this is a star
Pol I this is a star E. coli (mutA) this is a star
Nitrous acid this is a star Formic acid this is a star
Hydrazine this is a star EMS this is a star
 
Reference: Verma R, Schwaneberg U, Roccatano D 2012. MAP2.03D: A sequence/structure based server for protein engineering  
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